The DiagCor GenoFlow HPV Array Test is a PCR-DNA hybridization assay that uses “Flow-through” hybridization technology to detect 33 different human papillomavirus (HPV) genotypes from DNA isolated from cervical cells collected and stored in liquid-based cytology preservatives.
The DiagCor GenoFlow HPV Array Test is an efficient test in detecting the presence of HPV DNA. The assay uses specific nucleic acid primer sets for sensitive amplification of HPV DNA from the L1 gene sequence of the HPV genome. After PCR amplification, PCR amplicons are detected with the highly efficient “Flow-through” hybridization technique. The whole procedure, including DNA extraction, PCR amplification, hybridization, and data interpretation, takes about 3 hours to complete, with 1 hour hands-on time only.
The DiagCor GenoFlow HPV Array Test is one of the most comprehensive assays for HPV genotyping. It covers 33 of the most common HPV genotypes, including 18 high-risk HPV (HR-HPV) genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 81 and 82, and 15 low-risk HPV (LR-HPV) genotypes: 6, 11, 26, 40, 42, 43, 44, 54, 55, 57, 61, 70, 71, 72 and 84. The test kit can also detect rarer HPV genotypes outside of those listed above with its “universal probe”.
The assay also provides an internal control which detects a sequence in the human beta-globin gene to monitor sample adequacy, DNA extraction, PCR inhibition and PCR amplification. This minimizes the chance of false negative results.
For a standard molecular biology laboratory, the additional instrumentation requirement to use the test is minimal. Molecular biology laboratories that have a thermal cycler can begin using the test by simply obtaining the “Flow-through” hybridization device. No additional data interpretation device is required.