Principle of "Flow-through" Hybridization Technology

"Flow-through" Hybridization Technology provides the platform for rapid analyses of any analyte in the Solution-Porous-Matrix System. This is exemplified by the DNA hybridization: It is characterized by active approach to facilitate the annealing reaction between the sample molecules (the targets) and the capturing molecules (probes), which occur on three-dimensional porous matrixes rather than planar surfaces.
The annealing of two complimentary DNA single strands is a very fast kinetic process when the two strands collide. However in the two-phase conventional hybridization system, the target molecules must move toward the probes fixed in the solid matrix during hybridization. Hence this diffusion through solid matrix - a slow process - is the rate-limiting step and therefore the incubation step usually takes hours or even overnight in order to achieve optimal result.