“DNA-based assay for quick screening of 16 high-risk HPV subtypes on liquid-based cytology cervical specimens”
The DiagCor GenoFlow HPV-HR (HPV-High Risk) Test is a PCR-DNA hybridization assay that uses “Flow-through” hybridization technology to detect 16 HPV high-risk subtypes, with the capability to differentiate between type 16 and 18. The test uses DNA isolated from cervical cells collected and stored in liquid-based cytology preservatives.
The DiagCor GenoFlow HPV-HR Test is an efficient test in detecting the presence of HPV DNA. The assay uses specific nucleic acid primer sets for sensitive amplification of HPV DNA from the L1 gene sequence of the HPV genome. After PCR amplification, PCR amplicons are detected with the highly efficient “Flow-through” hybridization technique. The whole procedure, including DNA extraction, PCR amplification, hybridization, and data interpretation, takes about 3 hours to complete, with 1 hour hands-on time only.
The DiagCor GenoFlow HPV-HR Test is most suitable for screening application and can simultaneously screen 48 samples on DiagCor’s “Flow-through” hybridization device. The assay also provides an internal control which detects a sequence in the human beta-globin gene to monitor sample adequacy, DNA extraction, PCR inhibition and PCR amplification. This minimizes the chance of false negative results.
For a standard molecular biology laboratory, the additional instrumentation requirement to use the test is minimal. Molecular biology laboratories that have a thermal cycler can begin using the test by simply obtaining the “Flow-through” hybridization device. No additional data interpretation device is required.